Using the target and gene clusters previously computed, we cluster on the cluster averages. The hierarchical clustering is used to reorder the heatmaps.
library(magrittr)
library(tidyverse)
library(pheatmap)
library(bluster)
library(Matrix)
set.seed(20210818)
CLUSTERS_GENE_UNREG=c(9,12,13)
## input files
FILE_CLUSTERS_TARGETS="tbl/df_target_nnclusters_kd6_essential_ui10.csv"
FILE_CLUSTERS_GENES="tbl/df_gene_nnclusters_kd6_essential_ui10.csv"
RDS_DWUI="data/dwui/20221107-dwui-target-gene-kd6-essential.Rds"
RDS_ZDWUI="data/dwui/20221107-zdwui-target-gene-kd6-essential.Rds"
## output files
FILE_OUT_AVG_TARGET="img/heatmap-kd6-essential-avg-target-clusters.pdf"
FILE_OUT_AVG_GENE="img/heatmap-kd6-essential-avg-gene-clusters.pdf"
FILE_OUT_AVG_BOTH="img/heatmap-kd6-essential-avg-both-clusters.pdf"
FILE_OUT_FINAL="img/heatmap-kd6-essential-nnclusters-ordered.pdf"
## aesthetics
NCOLORS=100
COLORS_BWR <- colorRampPalette(c("blue", "white", "red"))(NCOLORS)
COLORS_MKY <- colorRampPalette(c("magenta", "black", "yellow"))(NCOLORS)
COLORS_YKM <- colorRampPalette(c("yellow", "black", "magenta"))(NCOLORS)
COLORS_RKG <- colorRampPalette(c("red", "black", "green"))(NCOLORS)
breaks_dwui <- seq(-0.5, 0.5, length.out=NCOLORS + 1)
breaks_zdwui <- seq(-4, 4, length.out=NCOLORS + 1)
breaks_zdwui_broad <- seq(-6, 6, length.out=NCOLORS + 1)
breaks_pca <- seq(-20, 20, length.out=NCOLORS + 1)
dwui_target_gene <- readRDS("data/dwui/20221107-dwui-target-gene-kd6-essential.Rds")
dwui_target_gene[is.na(dwui_target_gene)] <- 0
zdwui_target_gene <- readRDS("data/dwui/20221107-zdwui-target-gene-kd6-essential.Rds")
zdwui_target_gene[is.na(zdwui_target_gene)] <- 0
df_cluster_target <- read_csv(FILE_CLUSTERS_TARGETS) %>%
mutate(cluster_id=factor(cluster_id, levels=sort(unique(cluster_id))))
df_cluster_gene <- read_csv(FILE_CLUSTERS_GENES) %>%
mutate(cluster_id=factor(cluster_id, levels=sort(unique(cluster_id))))
sgid2gene <- df_cluster_target %>%
dplyr::select(sgID_AB, target_gene) %>%
distinct(sgID_AB, target_gene) %>%
deframe()
ens2gene <- df_cluster_gene %>%
dplyr::select(gene_id, gene_name) %>%
distinct(gene_id, gene_name) %>%
deframe()
convert_rownames <- function (mat, in2out) {
mat %>% set_rownames(in2out[rownames(.)])
}
convert_colnames <- function (mat, in2out) {
mat %>% set_colnames(in2out[colnames(.)])
}
idx_targets1 <- df_cluster_target %>%
arrange(cluster_id, target_gene) %$%
sgID_AB
idx_genes1 <- df_cluster_gene %>%
arrange(cluster_id, gene_name) %$%
gene_id
idx_genes_clean <- df_cluster_gene %>%
filter(!(cluster_id %in% CLUSTERS_GENE_UNREG)) %>%
arrange(cluster_id, gene_name) %$%
gene_id
I_cluster_target <- df_cluster_target %>%
dplyr::select(sgID_AB, cluster_id) %>%
deframe %>% fac2sparse
## cluster target means
M_cluster_target <- I_cluster_target %>% { . / rowSums(.) }
mzdwui_cluster_gene <- M_cluster_target[,idx_targets1] %*% zdwui_target_gene[idx_targets1,]
mzdwui_cluster_gene %<>% as.matrix
df_col_annots <- df_cluster_gene %>%
dplyr::select(gene_id, cluster_id) %>%
rename(gene_cluster=cluster_id) %>%
column_to_rownames("gene_id")
pheatmap(mzdwui_cluster_gene[, idx_genes_clean],
color=COLORS_BWR,
breaks=breaks_zdwui,
fontsize_col=1, fontsize_row=10,
annotation_col=df_col_annots,
show_colnames=FALSE, show_rownames=TRUE, scale='none',
cluster_rows=TRUE, cluster_cols=FALSE, clustering_method="complete",
cutree_rows=NA, cutree_cols=NA)
idx_clusters_targets <- c(14,18,11,16,13,9,12,2,5,15,1,6,8,3,7,4,10,17)
pheatmap(mzdwui_cluster_gene[idx_clusters_targets, idx_genes_clean],
color=COLORS_BWR,
breaks=breaks_zdwui,
fontsize_col=1, fontsize_row=10,
#annotation_row=df_row_annots,
annotation_col=df_col_annots,
show_colnames=FALSE, show_rownames=TRUE, scale='none',
cluster_rows=FALSE, cluster_cols=FALSE, clustering_method="complete",
#gaps_row=cumsum(table(clusters_targets_final)),
#gaps_col=cumsum(table(clusters_genes_final)) %>% `[`(!duplicated(.)),
cutree_rows=NA, cutree_cols=NA)
idx_targets2 <- df_cluster_target %>%
mutate(cluster_id=factor(cluster_id, levels=idx_clusters_targets)) %>%
arrange(cluster_id, target_gene) %$%
sgID_AB
df_row_annots <- df_cluster_target %>%
dplyr::select(sgID_AB, cluster_id) %>%
mutate(cluster_id=factor(cluster_id, levels=idx_clusters_targets)) %>%
rename(target_cluster=cluster_id) %>%
column_to_rownames("sgID_AB")
pheatmap(zdwui_target_gene[idx_targets2, idx_genes_clean],
color=COLORS_BWR,
breaks=breaks_zdwui,
fontsize_col=1, fontsize_row=10,
annotation_row=df_row_annots,
annotation_col=df_col_annots,
show_colnames=FALSE, show_rownames=FALSE, scale='none',
cluster_rows=FALSE, cluster_cols=FALSE, clustering_method="complete")
I_cluster_gene <- df_cluster_gene %>%
filter(!(cluster_id %in% CLUSTERS_GENE_UNREG)) %>%
dplyr::select(gene_id, cluster_id) %>%
deframe %>% fac2sparse
## cluster target means
M_cluster_gene <- I_cluster_gene %>% { . / rowSums(.) }
mzdwui_target_cluster <- zdwui_target_gene[,idx_genes_clean] %*% t(M_cluster_gene[,idx_genes_clean])
mzdwui_target_cluster %<>% as.matrix
pheatmap(mzdwui_target_cluster[idx_targets2,],
color=COLORS_BWR,
breaks=breaks_zdwui,
fontsize_col=10, fontsize_row=10,
show_colnames=TRUE, show_rownames=FALSE, scale='none',
angle_col=0, annotation_names_row=FALSE,
annotation_row=df_row_annots,
cluster_rows=FALSE, cluster_cols=TRUE, clustering_method="complete",
drop_levels=TRUE)
idx_clusters_genes <- as.character(c(20,16,7,8,1,3,18,2,5,10,15,19,14,11,4,6,17))
pheatmap(mzdwui_target_cluster[idx_targets2, idx_clusters_genes],
color=COLORS_BWR,
breaks=breaks_zdwui,
fontsize_col=10, fontsize_row=10,
show_colnames=TRUE, show_rownames=FALSE, scale='none',
annotation_row=df_row_annots,
cluster_rows=FALSE, cluster_cols=FALSE, clustering_method="complete",
drop_levels=TRUE)
idx_genes2 <- df_cluster_gene %>%
filter(!(cluster_id %in% CLUSTERS_GENE_UNREG)) %>%
mutate(cluster_id=factor(cluster_id, levels=idx_clusters_genes)) %>%
arrange(cluster_id, gene_name) %$%
gene_id
df_col_annots <- df_cluster_gene %>%
dplyr::select(gene_id, cluster_id) %>%
mutate(cluster_id=factor(cluster_id, levels=idx_clusters_genes)) %>%
rename(gene_cluster=cluster_id) %>%
column_to_rownames("gene_id")
gaps_col <- df_cluster_gene %>%
filter(!(cluster_id %in% CLUSTERS_GENE_UNREG)) %>%
mutate(cluster_id=factor(cluster_id, levels=idx_clusters_genes)) %>%
arrange(cluster_id, gene_name) %$%
table(cluster_id) %>%
cumsum
gaps_row <- df_cluster_target %>%
mutate(cluster_id=factor(cluster_id, levels=idx_clusters_targets)) %>%
arrange(cluster_id, target_gene) %$%
table(cluster_id) %>%
cumsum
pheatmap(zdwui_target_gene[idx_targets2, idx_genes2],
color=COLORS_BWR,
breaks=breaks_zdwui,
fontsize_col=1, fontsize_row=10,
annotation_row=df_row_annots,
annotation_col=df_col_annots,
show_colnames=FALSE, show_rownames=FALSE, scale='none',
annotation_names_row=FALSE, annotation_names_col=FALSE,
gaps_row=gaps_row,
gaps_col=gaps_col,
cluster_rows=FALSE, cluster_cols=FALSE, clustering_method="complete")
pheatmap(zdwui_target_gene[idx_targets2, idx_genes2],
color=COLORS_BWR,
breaks=breaks_zdwui,
fontsize_col=1, fontsize_row=1,
annotation_row=df_row_annots,
annotation_col=df_col_annots,
show_colnames=TRUE, show_rownames=TRUE, scale='none',
labels_row=sgid2gene[idx_targets2],
labels_col=ens2gene[idx_genes2],
annotation_names_row=FALSE, annotation_names_col=FALSE,
gaps_row=gaps_row,
gaps_col=gaps_col,
cluster_rows=FALSE, cluster_cols=FALSE,
filename=FILE_OUT_FINAL, width=16, height=16)
pheatmap(mzdwui_cluster_gene[, idx_genes2],
color=COLORS_BWR,
breaks=breaks_zdwui,
fontsize_col=1, fontsize_row=10,
annotation_col=df_col_annots,
annotation_names_row=FALSE, annotation_names_col=FALSE,
show_colnames=FALSE, show_rownames=TRUE, scale='none',
cluster_rows=TRUE, cluster_cols=FALSE, clustering_method="complete",
cutree_rows=NA, cutree_cols=NA,
filename=FILE_OUT_AVG_TARGET, width=8, height=4)
pheatmap(mzdwui_target_cluster[idx_targets2,],
color=COLORS_BWR,
breaks=breaks_zdwui,
fontsize_col=10, fontsize_row=10,
show_colnames=TRUE, show_rownames=FALSE, scale='none',
angle_col=0,
annotation_row=df_row_annots,
annotation_names_row=FALSE, annotation_names_col=FALSE,
cluster_rows=FALSE, cluster_cols=TRUE, clustering_method="complete",
drop_levels=TRUE,
filename=FILE_OUT_AVG_GENE, width=5, height=6)
mzdwui_cluster_cluster <- M_cluster_target[,idx_targets2] %*% mzdwui_target_cluster[idx_targets2,]
pheatmap(mzdwui_cluster_cluster,
color=COLORS_BWR,
breaks=breaks_zdwui,
fontsize_col=10, fontsize_row=10,
show_colnames=TRUE, show_rownames=TRUE, scale='none',
angle_col=0,
cluster_rows=TRUE, cluster_cols=TRUE, clustering_method="complete")
pheatmap(mzdwui_cluster_cluster,
color=COLORS_BWR,
breaks=breaks_zdwui,
fontsize_col=10, fontsize_row=10,
show_colnames=TRUE, show_rownames=TRUE, scale='none',
angle_col=0,
cluster_rows=TRUE, cluster_cols=TRUE, clustering_method="complete",
filename=FILE_OUT_AVG_BOTH, width=5, height=4)
## R version 4.1.1 (2021-08-10)
## Platform: x86_64-apple-darwin13.4.0 (64-bit)
## Running under: macOS Big Sur 10.16
##
## Matrix products: default
## BLAS/LAPACK: /Users/mfansler/miniconda3/envs/bioc_3_14/lib/libopenblasp-r0.3.18.dylib
##
## locale:
## [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
##
## attached base packages:
## [1] stats graphics grDevices utils datasets methods base
##
## other attached packages:
## [1] Matrix_1.3-4 bluster_1.4.0 pheatmap_1.0.12 forcats_0.5.1
## [5] stringr_1.4.0 dplyr_1.0.8 purrr_0.3.4 readr_2.1.1
## [9] tidyr_1.1.4 tibble_3.1.7 ggplot2_3.3.5 tidyverse_1.3.1
## [13] magrittr_2.0.3
##
## loaded via a namespace (and not attached):
## [1] httr_1.4.2 sass_0.4.0 bit64_4.0.5
## [4] vroom_1.5.7 jsonlite_1.7.2 modelr_0.1.8
## [7] bslib_0.3.1 assertthat_0.2.1 highr_0.9
## [10] stats4_4.1.1 cellranger_1.1.0 yaml_2.2.1
## [13] pillar_1.7.0 backports_1.4.0 lattice_0.20-45
## [16] glue_1.6.2 digest_0.6.29 RColorBrewer_1.1-2
## [19] rvest_1.0.2 colorspace_2.0-2 htmltools_0.5.2
## [22] pkgconfig_2.0.3 broom_0.8.0 haven_2.4.3
## [25] scales_1.1.1 tzdb_0.2.0 BiocParallel_1.28.0
## [28] generics_0.1.1 farver_2.1.0 ellipsis_0.3.2
## [31] withr_2.4.3 BiocGenerics_0.40.0 cli_3.3.0
## [34] crayon_1.4.2 readxl_1.3.1 evaluate_0.15
## [37] fs_1.5.2 fansi_0.5.0 xml2_1.3.3
## [40] tools_4.1.1 hms_1.1.1 lifecycle_1.0.1
## [43] S4Vectors_0.32.0 munsell_0.5.0 reprex_2.0.1
## [46] cluster_2.1.2 compiler_4.1.1 jquerylib_0.1.4
## [49] rlang_1.0.2 grid_4.1.1 BiocNeighbors_1.12.0
## [52] rstudioapi_0.13 igraph_1.2.9 rmarkdown_2.11
## [55] gtable_0.3.0 DBI_1.1.1 R6_2.5.1
## [58] lubridate_1.8.0 knitr_1.39 fastmap_1.1.0
## [61] bit_4.0.4 utf8_1.2.2 stringi_1.7.6
## [64] parallel_4.1.1 Rcpp_1.0.7 vctrs_0.4.1
## [67] dbplyr_2.1.1 tidyselect_1.1.1 xfun_0.30
## Conda Environment YAML
name: base
channels:
- conda-forge
- bioconda
- defaults
dependencies:
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- pysocks=1.7.1=pyha2e5f31_6
- python=3.9.13=h57e37ff_0_cpython
- python-dateutil=2.8.2=pyhd8ed1ab_0
- python-libarchive-c=4.0=py39h6e9494a_1
- python-tzdata=2021.5=pyhd8ed1ab_0
- python_abi=3.9=2_cp39
- pytz=2021.1=pyhd8ed1ab_0
- pytz-deprecation-shim=0.1.0.post0=py39h6e9494a_2
- pyyaml=5.4.1=py39h701faf5_3
- readline=8.1.2=h3899abd_0
- reproc=14.2.3=h0d85af4_0
- reproc-cpp=14.2.3=he49afe7_0
- requests=2.28.1=pyhd8ed1ab_1
- requests-oauthlib=1.3.0=pyh9f0ad1d_0
- rich=10.16.1=pyhd8ed1ab_0
- ripgrep=13.0.0=h244e342_0
- rsa=4.7.2=pyh44b312d_0
- rsync=3.2.7=ha1fed10_0
- ruamel.yaml=0.17.21=py39h63b48b0_1
- ruamel.yaml.clib=0.2.6=py39h63b48b0_1
- ruamel_yaml=0.15.80=py39h701faf5_1007
- s3transfer=0.6.0=pyhd8ed1ab_0
- scrypt=0.8.18=py39hbfd427f_4
- setuptools=65.3.0=pyhd8ed1ab_1
- six=1.16.0=pyh6c4a22f_0
- smartmontools=7.2=h940c156_0
- smmap=3.0.5=pyh44b312d_0
- soupsieve=2.3.1=pyhd8ed1ab_0
- sqlite=3.38.5=hd9f0692_0
- tapi=1100.0.11=h9ce4665_0
- tk=8.6.12=h5dbffcc_0
- toolz=0.11.1=py_0
- tornado=6.2=py39h701faf5_0
- tqdm=4.62.2=pyhd8ed1ab_0
- traitlets=5.1.0=pyhd8ed1ab_0
- typing_extensions=3.10.0.0=pyha770c72_0
- tzdata=2021e=he74cb21_0
- tzlocal=4.2=py39h6e9494a_1
- urllib3=1.26.6=pyhd8ed1ab_0
- vsts-python-api=0.1.22=py_0
- watchgod=0.7=pyhd8ed1ab_0
- wcwidth=0.2.5=pyh9f0ad1d_2
- wget=1.20.3=h52ee1ee_1
- wheel=0.37.0=pyhd8ed1ab_1
- wrapt=1.14.1=py39h701faf5_0
- xxhash=0.8.0=h35c211d_3
- xz=5.2.5=haf1e3a3_1
- yaml=0.2.5=haf1e3a3_0
- yaml-cpp=0.7.0=hb486fe8_1
- zipp=3.5.0=pyhd8ed1ab_0
- zlib=1.2.13=hfd90126_4
- zstd=1.5.2=hfa58983_4
- pip:
- pyopenssl==20.0.1
prefix: /Users/mfansler/miniconda3